Point-of-care diagnostics
•Optical Bioassay
•Optical Biochip
•Fluorescent spectroscopy applied to bioassay
•Chemical functionalization of surfaces
•Immobilization of biological recognition element (antibody, DNA/RNA sequence, polipeptide, etc)
For more detailed information please consult the P4L database.
Case study:
Fluorescence-based platform for biosensing
Optical platform based on fluorescence anisotropy
-Stand alone optical platform for the analysis of biological fluids
- Simultaneous measurements of more than one analyte
- Automated processing of the biological sample (dilution and mixing)
Optical Biochip
- Plastic (PMMA) chip
- Thirteen microchannels (50 μm high, 600 μm width, 10 mm long)
- Sensing layer immobilised on the top surface of the microchannel
- Comb-shape of the top of the chip which implies that the fluorescence is guided along plastic waveguides
Application to sespis diagnosis
-Sandwich-immunoassay for C-reactive protein in serum and plasma
-Sandwich- immunoassay for procalcitonin in serum and plasma
-Covalent immobilisation of the selective capture antibody
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