Point-of-care diagnostics

•Optical Bioassay

•Optical Biochip

•Fluorescent spectroscopy applied to bioassay

•Chemical functionalization of surfaces

•Immobilization of biological recognition element (antibody, DNA/RNA sequence, polipeptide, etc)

For more detailed information please consult the P4L database.

Case study: Fluorescence-based platform for biosensing

Optical platform based on fluorescence anisotropy

-Stand alone optical platform for the analysis of biological fluids

- Simultaneous measurements of more than one analyte

- Automated processing of the biological sample (dilution and mixing)

Optical platform based on fluorescence anisotropy

Optical Biochip

- Plastic (PMMA) chip

- Thirteen microchannels (50 μm high, 600 μm width, 10 mm long)

- Sensing layer immobilised on the top surface of the microchannel

- Comb-shape of the top of the chip which implies that the fluorescence is guided along plastic waveguides

Optical Biochip

Application to sespis diagnosis

-Sandwich-immunoassay for C-reactive protein in serum and plasma

-Sandwich- immunoassay for procalcitonin in serum and plasma

-Covalent immobilisation of the selective capture antibody

Application to sespis diagnosis